Figure 7. ChIP analysis of BRG1 binding at cellular promoters.
The parent as well as representative cell lines from the vector and BRM RNAi stable clones were harvested and protein was extracted for ChIPs assays as described in the Material and Methods. ChIPs assays were performed using antibodies directed against 3 core members of the SWI/SNF complex including hSNF5, BAF155 and BRG-1. ChIP analyses were carried out at 3 promoters where the SWI/SNF complex had previously shown binding. Normal rabbit IgG served as a control for non-specific antibody binding. Values are the mean of triplicates.