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. Author manuscript; available in PMC: 2015 May 17.
Published in final edited form as: Eur J Neurosci. 2014 Mar 13;39(10):1572–1585. doi: 10.1111/ejn.12517

Fig. 4.

Fig. 4

HVA calcium current comes first during pupal life. MN5 calcium current as elicited by 200-ms depolarizing voltage steps from −90 to +20 mV in 10-mV increments from a holding potential of −90 mV. Leak was subtracted offline. HVA calcium currents were detectable from P4 on (A), and could reliably be evoked from P5 on through pupal life (B–F). With increasing complexity of the MN5 dendritic tree, the staircase-like appearance of HVA calcium current occurred (C–F). (G) IV-plot for HVA calcium currents at pupal stages P5, P8, P10, P12 and P15. At all stages HVA calcium current activated between −40 and −30 mV, with maximum amplitudes at 0 mV (P5, P12, P15) or at −10 mV (P8, P10), respectively (G). HVA calcium current was completely blocked by application of the cacophony calcium channel blocker PLTXII at a 20 nM concentration (H). The mean calcium current amplitude increased significantly between P5 and P8 (I, gray circles, P < 0.05, ANOVA with LSD pair-wise post hoc comparisons, for single values see black diamonds). Errors represent SEM Statistically significant difference was assumed at *P < 0.05.