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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Apr 7;1852(7):1410–1419. doi: 10.1016/j.bbadis.2015.03.012

FIGURE 5. NF-κB dysregulation after membrane depolarization in mdx myotubes is mediated by excessive ROS production.

FIGURE 5

A. NF- κB activity induced by H2O2 in wt and mdx myotubes. Cells were stimulated with different concentrations of exogenous H2O2. Luciferase activity was determined after 12h (n=3). B. NOX2 inhibition (apocynin) blunted depolarization-induced changes in NF-κB activity in both wt and mdx myotubes (n=3). C. NOX2 inhibition blocked depolarization-induced changes in IL-6 expression in both wt and mdx myotubes (n=3). D. BAPTA-AM (30 µM) blocked depolarization-induced changes in IL-6 expression in both wt myotubes *=P<0.05, **=P<0.01, ***=P<0.001 (indicated or versus basal wt value), and ns, no significant difference.