Figure 3. PGE₂ does not decrease gene expression of proteins involved in NLRP3 inflammasome complex formation.

(A-E) primary MDM were treated with/without LPS (100 ng/ml) for 4 h in complete medium, followed by treatment with PGE₂ (0.1 μM) or vehicle (EtOH) for indicated time in serum-free medium. At each time point, cells were lysed and total RNA was extracted. Gene expression was assessed using RT PCR, normalized to GAPDH transcripts and represented as a relative quantification (RQ) compared with vehicle treated cells from the first time-point. Data represent the mean ± SEM from 4 independent experiments from 4 healthy donors, each performed in duplicate. * P < .05 as indicated, as assessed by Kruskal-Wallis ANOVA on ranks, followed by Dunn's post hoc test.