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. Author manuscript; available in PMC: 2016 Jul 28.
Published in final edited form as: Cancer Lett. 2015 Apr 24;363(2):166–175. doi: 10.1016/j.canlet.2015.04.017

Fig. 4.

Fig. 4

Caspase-8 is required for FLLL12-induced apoptosis. (A) A549 and H1299 cells were treated with FLLL12 for 24 h and expression of caspase-8, Bid and tBid were analyzed. (B) A549 and H1299 cells were treated with FLLL12 for 24 h and caspase-8 activity was determined by Caspase Glo 8 kit. (C) Expression of caspase-8 was ablated in A549 and H1299 cells by siRNA and apoptosis was quantified after treatment with FLLL12 for 24 h. (D) A549 and H1299 cells with ablated caspase-8 were treated as in (C) and expression of caspase-8, cleaved caspase-3, cleaved PARP and Bid were analyzed in whole cell lysates. (E) Expression of Bid was ablated in A549 and H1299 cells and apoptosis was quantified as in (C). (F) A549 and H1299 cells with ablated Bid were treated as in (E) and expression of Bid, cleaved caspase-3 and cleaved PARP were analyzed in whole cell lysates.