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. Author manuscript; available in PMC: 2016 Jun 19.
Published in final edited form as: J Mol Biol. 2015 Apr 11;427(12):2220–2228. doi: 10.1016/j.jmb.2015.04.002

Figure 4. Crystallographic and spectroscopic analysis of IspG with the epoxide substrate 5.

Figure 4

(a) The structure (PDB ID code: 4S3C) was obtained after incubation of oxidized IspG with 5 for 24 hours. It reveals an open epoxide ring and an overall geometry resembling Figure 2B (contour level of the 2FoFc electron density = 1.0 sigma). (b) The 9 GHz EPR spectra of IspG + 5. The top spectrum was obtained in the presence of dithionite and corresponds to the proposed ferraoxetane intermediate and the HMBPP product. The other spectra were obtained in the absence of any reducing agent and do not indicate the formation of any radical species.