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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Apr 15;1852(7):1531–1539. doi: 10.1016/j.bbadis.2015.04.008

Figure 1. Early Complex I assembly intermediate in ND6 mutant cells.

Figure 1

A) shows the presence of a >950KDa putative Complex I band in A9 and ND6 mutant 4A cells at 0h after [35]S methionine labeling. The >950KDa band can also be seen in 4A cells at 24 h, while in A9 cells, the assembled Complex I band along with supercomplexes is seen. B) the chase experiment shows the gradual disappearance of the >950KDa Complex I intermediate band in 4A cells over time. C) [35]S labeled pulse chase of wild type A9 cells showing the >950KDa complex at early times and the assembled Complex I, indicated as Complex I*, and supercomplexes at later time point. Complexes III, IV and V are marked for molecular weight reference.