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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Apr 15;1852(7):1531–1539. doi: 10.1016/j.bbadis.2015.04.008

Figure 5. Lack of assembly of Complex I in ND4 mutant cells.

Figure 5

A) shows the 1D BNPAGE of KB30 and ND4 mutant C4T cells at the 0 h and 24 h time points of [35]S labeling. An early Complex I band is seen in KB30 at 0 h but not in C4T cells. B) Shows the 2D SDS PAGE of C4T cells and D) KB30 cells at 0 h time point after [35]S labeling and C) shows the 2DSDS PAGE of C4T cells and E) KB30 cells at the 24 h time point after [35]S labeling. The cartoon shows the presence of the ~400KDa and ~700KDa subcomplexes seen in the 2DSDS profile in ND4 mutant cells.