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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Immunol. 2015 Apr 17;194(11):5407–5416. doi: 10.4049/jimmunol.1402277

Fig. 1. Atg7Δ mice presents with spontaneous lung inflammation.

Fig. 1

Bone marrow-derived macrophages (BMDM) from wild type (Atg7f/f) or Atg7Δ mice were analyzed by immunoblotting (A). Lung tissues from two (B), six (C) or ten (D) month old mice were analyzed by hematoxylin and eosin (H&E) staining. Flow cytometry analysis of single cell suspensions of the lungs was done using the pan leukocytic marker anti-CD45 (E) or anti-Ly6G (F). Quantification graphs are shown. Bronchoalveolar lavage (BAL) from 2–4 months old Atg7Δ or control Atg7f/f mice was analyzed for total cell count (G) or differential cell count (H). Mϕ, macrophages; Lymph, lymphocytes; PMN, polymorphonuclear neutrophils. Data are mean ± SEM, n=3 to 8 mice/genotype, *p<0.05, ** p<0.01 Scale bar, 50 μm.