Table 3.
Clonal analysis of HLA-B*42:01-restricted TL9-specific CD8+ T-cell populations.
| Subject (ddmmyy) | Epitope | CD8+Tet+ | pVL | CD4 | TRBV | CDR3 | TRBJ | Freq (%) |
|---|---|---|---|---|---|---|---|---|
| N021 (040211) | TL9 | 1.4 | 8249 | 510 | 4-1 | CASSQEGGGQGQPQH | 1-5 | 60.00 |
| HLA-A*30:01/34:02 | 5-6 | CASSLATDGYT | 1-2 | 9.41 | ||||
| HLA-B*42:01/44:03 | 4-1 | CASSQEGGGDGQPQH | 1-5 | 8.24 | ||||
| HLA-C*07:01/17:01 | 12-3/4 | CASSFGLDEAF | 1-1 | 5.88 | ||||
| 4-1 | CASSQEGGGEGQPQH | 1-5 | 4.71 | |||||
| 12-3/4 | CASSFSKNTEAF | 1-1 | 3.53 | |||||
| 5-5 | CASSLEGTSGPQETQY | 2-5 | 2.35 | |||||
| 12-3/4 | CASSVGPNEQF | 2-1 | 2.35 | |||||
| 4-1 | CASSQEGGGDGQPPH | 1-5 | 1.18 | |||||
| 4-1 | CASRQEGGGDGQPQH | 1-5 | 1.18 | |||||
| 4-1 | CAPTPEGGGQGQPQH | 1-5 | 1.18 | |||||
| Number of cells sorted | 5000 | count | (85) | |||||
| R014 (130907) | TL9 | 2.7 | 1177 | 300 | 12-3/4 | CASSLGPTEAF | 1-1 | 31.25 |
| HLA-A*30:01/33:01 | 12-3/4 | CASSLDPEKGAF | 1-1 | 21.25 | ||||
| HLA-B*42:01/57:03 | 12-3/4 | CASSLGLNTIY | 1-3 | 20.00 | ||||
| HLA-C*17:01/18:01 | 12-3/4 | CASSFSKNTEAF | 1-1 | 10.00 | ||||
| 12-3/4 | CASSLGVNTIY | 1-3 | 7.50 | |||||
| 12-3/4 | CASSQGPTEAF | 1-1 | 6.25 | |||||
| 6-2/3 | CATHAGTGELF | 2-2 | 2.50 | |||||
| 12-3/4 | CASSLSFTEAF | 1-1 | 1.25 | |||||
| Number of cells sorted | 4701 | count | (80) | |||||
| H022 (170807) | TL9 | 6.1 | 125688 | 430 | 12-3/4 | CASSLNGADGYT | 1-2 | 76.83 |
| HLA-A*02:02/03:01 | 12-3/4 | CASSLGLNTIY | 1-3 | 13.41 | ||||
| HLA-B*08:01/42:01 | 7-9 | CASSSQTSGLFANTGELF | 2-2 | 9.76 | ||||
| HLA-C*07:02/17:01 | count | (82) | ||||||
| Number of cells sorted | 5000 | |||||||
| R081 (070909) | TL9 | 1.1 | 503 | 130 | 12-3/4 | CASSLGANTIY | 1-3 | 52.50 |
| HLA-A*30:01/30:02 | 5-1 | CASSLAFGTSGGEQY | 2-7 | 11.25 | ||||
| HLA-B*35:01/42:01 | 4-1 | CASSQEGGGGGQPQH | 1-5 | 11.25 | ||||
| HLA-C*04:01/17:01 | 5-1 | CASSLSDVSWNTEAF | 1-1 | 8.75 | ||||
| 12-3/4 | CASSREGYSNQPQH | 1-5 | 3.75 | |||||
| 12-3/4 | CASSLSKNTEAF | 1-1 | 2.50 | |||||
| 12-3/4 | CASSPGNTEAF | 1-1 | 2.50 | |||||
| 12-3/4 | CASRDPYEQY | 2-7 | 2.50 | |||||
| 12-3/4 | CASDKGTGNYGYT | 1-2 | 1.25 | |||||
| 12-3/4 | CASSHSKNTEAF | 1-1 | 1.25 | |||||
| 12-3/4 | CASSFGGTTEAF | 1-1 | 1.25 | |||||
| 12-3/4 | CASGLGANTIY | 1-3 | 1.25 | |||||
| Number of cells sorted | 2008 | count | (80) | |||||
| N033 (100707) | TL9 | 3.7 | 415 | 620 | 7-9 | CASSSTITGMGDSGNTIY | 1-3 | 96.63 |
| HLA-A*30:01/36:01 | 7-9 | CTSSSTITGMGDSGNTIY | 1-3 | 1.12 | ||||
| HLA-B*42:01/53:01 | 7-9 | CASSSTITGMGVSGNTIY | 1-3 | 1.12 | ||||
| HLA-C*04:01/17:01 | 7-9 | CASSSAITGMGDSGNTIY | 1-3 | 1.12 | ||||
| Number of cells sorted | 5000 | count | (89) | |||||
| N086 (181208) | TL9 | 0.7 | 2794 | 370 | 6-1 | CASRASTGSGNTIY | 1-3 | 93.33 |
| HLA-A*33:03/33:03 | 27 | CASSLRHLASDYNSPLH | 1-5 | 2.22 | ||||
| HLA-B*42:01/53:01 | 6-1 | CAGRASTGSGNTIY | 1-3 | 2.22 | ||||
| HLA-C*03:02/04:01 | 6-1 | CASRARRPSNTIY | 1-3 | 2.22 | ||||
| Number of cells sorted | 4340 | count | (45) | |||||
| N106 (030310) | TL9 | 1.8 | 14014 | 350 | 5-5 | CASSLVFGTAGGQQF | 2-1 | 63.10 |
| HLA-A*02:01/02:05 | 12-3/4 | CASSFSKNTEAF | 1-1 | 33.33 | ||||
| HLA-B*42:01/53:01 | 6-5 | CASSWTETGELF | 2-2 | 2.38 | ||||
| HLA-C*04:01/17:01 | 5-5 | CTSSLVFGTAGGRQF | 2-1 | 1.19 | ||||
| Number of cells sorted | 4160 | count | (84) | |||||
| SK191 | TL9 | 2.7 | 76900 | 12-3/4 | CASSFSKNTEAF | 1-1 | 80.95 | |
| HLA-A*23:01/30:01 | 4-1 | CASSQEGGGQGQPQH | 1-5 | 5.95 | ||||
| HLA-B*08:01/42:01 | 5-1 | CASSLMGASGANVLT | 2-6 | 4.76 | ||||
| HLA-C*16:01/17:01 | 12-3/4 | CASSLGANTIY | 1-3 | 4.76 | ||||
| 12-3/4 | CASSLSKNTEAF | 1-1 | 2.38 | |||||
| 29-1 | CSVRTHQGPTNEKLF | 1-4 | 1.19 | |||||
| Number of cells sorted | 3674 | count | (84) |