Male CD36 −/− mice at eight weeks of age and age-matched wild type littermate controls were fed a low fat (10% kcal as fat) or a high fat diet (60% kcal as fat) for 16 weeks. After that, mice were harvested and kidney tissues were collected. Apoptotic cell number was calculated after tunel staining (A). The scale bar represents 50 µm. In addition, glomerular caspase 3 protein expression (B) and caspase 3 activity (C) were determined by using immunochemical staining and caspase-3 colorimetric assay kit, respectively. Data are presented as mean ± SE (n = 6 mice/group). *, P <0.05 vs. WT/lean group; #, P <0.05 vs. WT/obese group.