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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Mar 31;1852(7):1323–1333. doi: 10.1016/j.bbadis.2015.03.010

Figure 3. FFA treatment stimulated TSP1 expression in podocyte at the transcriptional level.

Figure 3

FFA treated human podocytes were harvested for analyzing TSP1 expression in mRNA (A) and protein (B) levels by Real-time PCR and Western blot assay, respectively. TSP1 protein levels in the conditioned media (CM) were determined by immunoblotting. The ponceau S staining was shown as loading control (C). The effect of FFA on TSP1 mRNA stability was determined by adding actinomycin D to inhibit de novo RNA synthesis after 24-h FFA treatment (D) and the effect of FFA on TSP1 transcriptional activity was tested by transfection with a mouse full-length TSP1 promoter-luciferase before 24-h FFA treatment (E). Data are presented as mean ± SE (n = 3 individual experiments). *, P <0.05 vs. control group; #, P <0.05 vs. 25 µM group.