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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Mar 31;1852(7):1323–1333. doi: 10.1016/j.bbadis.2015.03.010

Figure 6. TSP1 treatment induced podocyte apoptosis and functional change through a TGF-β independent pathway.

Figure 6

Human podocytes were treated with TSP1 (1µg/ml) for 24 h. Then Annexin V/PI staining for podocytes (A) and podocyte BSA filter assay (B) were performed. Human podocytes were pretreated with anti-TGF-β 1−3 antibody (15µg/ml) or control IgG for 30 minutes and then treated with TSP1 for 24 hours. After treatment, apoptotic cells (C) and BSA filter assay (D) were analyzed. Data are presented as mean ± SE (n = 3 individual experiments). *, P <0.05 and **, P<0.01 vs. control group