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. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: Clin Cancer Res. 2015 Feb 23;21(10):2338–2347. doi: 10.1158/1078-0432.CCR-14-3000

Figure 5. Effects of GO-203/NPs on breast cancer cell self-renewal.

Figure 5

A. The indicated breast cancer cells were seeded at 1000 cells/well in 6-well plates and left untreated (Control) or treated with 7.5 µM GO-203/NPs or an equal amount of empty NPs on day 0. After 3 days, NP containing media was replaced with fresh media. Colonies were stained with crystal violet on day 20 after treatment (upper panels). Colony number (>30 cells) is expressed as the mean+SD of three replicates (lower panel). B. MDA-MD-468 cells were left untreated (Control) or treated with 7.5 µM GO-203/NPs or CP-2/NPs once on day 0. The cells were then plated at 2000 cells/well in sphere culture on day 2. Representative images are shown for the indicated MDA-MB-468 cells grown for 5 days post-treatment in sphere culture (upper panels). Bar represents 100 microns. The percentage SFE is expressed as the mean±SD of three determinations (lower panel). C. MCF-7 cells were left untreated (Control) or treated with 7.5 µM GO-203/NPs or CP-2/NPs once on day 0. The cells were then plated at 2000 cells/well in sphere culture on day 2. Representative images are shown for the indicated MCF-7 cells grown for 5 days post-treatment in sphere culture (upper panels). Bar represents 100 microns. The percentage SFE is expressed as the mean±SD of three determinations (lower panel).

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