Table 1.

Purification of secreted laccase from the 72 h culture supernatant.

Step*	Volume (mL)	Protein (mg·mL−1)	Activity (U·mL−1)	Sp. act. (U·mg−1)	Yield (%)	Purification (fold)
Crude supernatant	1000	0.42	2.56	6.10	100	1
(NH4)2SO4 fractionation	12	1.26	30.43	24.15	14.3	3.96
Ni-NTA affinity chromatography	5	1.59	43.22	27.18	8.4	4.46

*Data are values obtained in triplicate assays with variations of <5%.