Table 1.
Primers and PCR conditions.
| Transcript and primers | PCR conditions | |||
|---|---|---|---|---|
| Specific first exon forward primers | ||||
| NESP55 (Accession no. NMJH6592) | ||||
| Round 1 | 5′‐GAAGAGTCGAAGGAGCCCAAG‐3′ | 95°C × 6 minutes; 95°C × 30 seconds, 57°C × 30 seconds, 72°C | ||
| Round 2 | 5′‐ GTCACTAATGGAGGACGCCGT‐3′ | × 1 minute 45 seconds × 35 cycles; 72°C × 10 minutes | ||
| XLαs (Accession no. NM_001077490) | ||||
| Round 1 | 5′‐GGATGCCTCCGCTGGTTTCAGCATCG‐3′ | 95°C × 6 minutes; 95°C × 30 seconds, 57°C × 30 seconds, 72°C | ||
| Round 2 | 5′‐CTTTCTCGTGCAAGCCTTC‐3′ | × 1 minute 45 seconds × 35 cycles; 72°C × 10 minutes | ||
| Gαs (Accession no. NM_000516) | ||||
| Round 1 | 5′‐ATGGGCTGCCTCGGGAACAGT‐3′ | 95°C × 3 minutes; 94°C × 30 seconds, 66°C × 2 minutes, × 39 cycles; 68°C × 5 minutes | ||
| Common downstream reverse primers | Product size (bp) | |||
| Exon 6 | NESP55 | XLαs | Gαs | |
| Round 1 | 5′‐TTCGTAGCAGGCACGCACTCC‐3′ | 482 | 616 | 451 |
| Round 2 | 5′‐GCCTTGGCATGCTCATAGA‐3′ | 329 | 517 | 416 |
| Exon 8 | ||||
| Round 1 | 5′‐GTCCACCTGGAACTTGGTCTC‐3′ | 635 | 769 | 604 |
| Round 2 | 5′‐CCAGAAGTCAGGACACGGCAG‐3′ | 489 | 677 | 576 |
| Exon 13 | ||||
| Round 1 | 5′‐AGAGCAGCTCGTACTGACGAAG‐3′ | 1,172 | 1,306 | 1,141 |
| Round 2 | 5′‐GCGCTGAATGATGTCACGGC‐3′ | 1,027 | 1,215 | 1,115 |