Figure 3.

Genes associated with rs763035 are present in rosacea skin. (a) Rosacea skin from an individual of European descent and heterozygous for the minor allele at rs763035 immunostained with human leukocyte antigen-DRA antibody with a strong signal in the perifollicular inflammatory infiltrate (black and white arrow), epidermal Langerhans cells (single black arrow), and endothelial cells (double arrows). (b) Rosacea skin from the same individual as a and b immunostained with BTNL2 antibody showed mild–moderate staining in keratinocytes, perifollicular infiltrate (black and white arrow), and endothelial cells (double arrow). (c) Normal skin from the same individual stains with human leukocyte antigen-DRA antibody but lacks perifollicular infiltrate, numerous epidermal Langerhans cells, and dilated blood vessels seen in rosacea skin. (d) Normal skin from the same individual stained with BTNL2 antibody in keratinocytes but does not contain inflammatory infiltrate or dilated blood vessels. Negative controls were performed for all the tissues above by omitting either human leukocyte antigen-DRA or BTNL2 primary antibodies, and these displayed no staining (data not shown). Scale bar=100 μm.