Figure 5. C2H2 motifs relate to DBD similarity and to the recognition code.
Left, ClustalW phylogram of C2H2 zinc finger (ZF) amino acid sequences with corresponding motifs. Right, examples in which motifs predicted by the ZF recognition code are compared to changes in DNA sequences preferred by paralogous C2H2 ZF TFs. Cartoon shows individual C2H2 ZFs and their specificity residues. Dashed lines correspond to 4-base subsites predicted from the recognition code.
Figure 5—figure supplement 1. Comparison of C2H2 ZF recognition model with motifs derived PBM.
Motif correlations between PBM derived motifs and ZF-model based predictions for TFs with both typical and atypical (A) linker lengths between ZF modules that are longer than 6 amino acids or shorter than 4 amino acids (B) zinc coordinating cysteine or histidine structural motifs and (C) differing length of the ZF array. Examples of recognition code predictions (sequence logos) for both typical and atypical TFs are compared with PBM motifs for each case. The p-values shown are estimated from Student's t-test. The number of TFs in each boxplot is shown above in parentheses.