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Optimized multiplex PCR for both R. solanacearum and X. Perforans. Lanes 1 and 2 amplification of R. solanacearum annealing temperature at 57 and 61 °C. Lanes 3 and 4 amplification of X. perforans annealing temperature at 58 and 64 °C. Lanes 5–7 multiplex PCR of R. solanacearum and X. perforans 61 ± 1 °C. 50 bp (M) Gene ladder
