Table 1.
Mixed culture, isolation of R. solanacearum and X. perforans from different sources and their reaction to multiplex PCR
| Source | Isolates of R. solanacearum | Isolates of X. perforans | Multiplex PCR | |
|---|---|---|---|---|
| R. solanacearum | X. perforans | |||
| Soil | RS 1 | XP 1 | + | + |
| Soil | RS 2 | XP 2 | + | + |
| Soil | RS 3 | XP 3 | + | + |
| Soil | RS 4 | XP 4 | + | + |
| Soil | RS 5 | XP 5 | + | + |
| Plant material | RS 6 | XP 6 | + | + |
| Plant material | RS 7 | XP 7 | + | + |
| Plant material | RS 8 | XP 8 | + | + |
| Plant material | RS 9 | XP 9 | + | + |
| Plant material | RS 10 | XP 10 | + | + |
| Seed | RS 11 | XP 11 | + | + |
| Seed | RS 12 | XP 12 | + | + |
| Seed | RS 13 | XP 13 | + | + |
| Seed | RS 14 | XP 14 | + | + |
| Seed | RS 15 | XP 15 | + | + |
| Seed | RS 16 | XP 16 | + | + |
| Seed | RS 17 | XP 17 | + | + |
| Seed | RS 18 | XP 18 | + | + |
| Seed | RS 19 | XP 19 | + | + |
| Seed | RS 20 | XP 20 | + | + |
| Seed | Cmm | Cmm | – | – |
| Seed | PS | PS | – | – |
RS 1–RS 5. Bacterial isolates isolated from serial dilution method, RS 6–RS 10. Bacterial isolates isolated from direct plating method of plant materials (stem), RS 11–Rs 15. Bacterial isolates isolated from direct plating method of seeds, RS 16–RS 20. Bacterial isolates isolated from liquid assay method for seeds
XP 1–XP 5. Bacterial isolates isolated from serial dilution method, XP 6–XP 10. Bacterial isolates isolated from direct plating method of plant materials (leaves), XP 11–XP 15. Bacterial isolates isolated from direct plating method of seeds, XP16–XP 20. Bacterial isolates isolated from liquid assay method for seeds
Cmm. Clavibacter michiganensis subsp. michiganensis and PS. Pseudomonas fluorescens bacterial isolate isolated from direct plating method of seed and soil, respectively