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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Nat Cell Biol. 2015 Apr 27;17(5):615–626. doi: 10.1038/ncb3160

Figure 6.

Figure 6

YAP is expressed in the nucleus of pancreatic MPCs, and shows co-occupancy with TEAD1 at MPC enhancers. (a) YAP is detected in the nucleus of PDX1+ in vivo MPCs from human Carnegie Stage 18 pancreas. (b) In 10 weeks post-conception (WPC) human pancreas YAP expression is strong in nuclei of PDX1+ progenitors, but shows markedly diminished signal intensity in NGN3+ progenitors (white arrow). Image depicts 5 cells in human embryonic pancreas 10 WPC. (c) Yap is detected in the nucleus of Sox9+ MPCs from mouse E12.5 embryonic pancreas (white arrow), whereas Yap is diffuse or absent in Ngn3+ endocrine progenitor cells (hollow arrowheads). (d) YAP is excluded from the nucleus in hESCs-derived pancreatic NGN3+ progenitor cells (hollow arrowheads). (e) ChIP-qPCR analysis of YAP occupancy in chromatin from in vitro MPCs shows that TEAD1-bound regions are often co-bound by YAP.