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Effect of PGH2 level on reaction rate in TXAS wild-type and variants. Continuous assays contained 5 nM of recombinant TXAS. Lines represent fits of the data to the Michaelis- Menten equation. Filled circles, wild-type TXAS; open circles, K258E; filled triangles, L357V; open triangles, Q417E; filled squares, E450K, and open squares, T451N. Data shown is from a representative set of experiments
