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. Author manuscript; available in PMC: 2015 May 18.
Published in final edited form as: J Biomol Screen. 2011 Feb;16(2):183–191. doi: 10.1177/1087057110389040

Figure 6. LOPAC hits concentration response curves (CRCs) and radioligand receptor binding assay.

Figure 6

A) The compound hits were titrated starting from 50 µM, with 3-fold serial dilution steps for 10 points. Each point on a curve is displaying the average of three results along with error bars. B) Radioligand competition assays were done using purified GST-RORα-LBD as protein and 3nM of [3H]-25-hydroxycholesterol as radioligand in the assay buffer as mentioned in Materials and Methods. The competition assay was performed for these compounds at 8 different concentrations starting from 10µM and 3-fold subsequent dilutions to 3nM. Data shown are representative results from the experiment performed in triplicates.