Fig. 4.
Transport assay showing uptake of radioactive UDP-GlcNAc into purified rat heart mitochondria and mitoplasts in the presence of different substrates and inhibitors. (A) Incorporation of time-dependent UDP-[3H]GlcNAc into purified mitochondria and its effect on presence of detergent, UTP, UMP, FCCP, ATP, and cyclosporine A (n = 3). (B) Immunoblot on mitoplast preparations showing intact inner mitochondrial membrane and ruptured outer membrane. (C) Uptake assay on mitoplasts showing efficient uptake on only mitoplast prep but reduced uptake on mitoplasts treated with either detergent or streptolysin O (n = 3). (D) Effect of major mitochondrial transport inhibitors on UDP-GlcNAc transport (n = 3). (t test was carried out for mito samples vs. different treatments with P < 0.05 considered significant.)