Fig. 4.

Effects of Mrc1 and Rad9 on pol2-4 and pol2-M644G phenotypes. (A) Viability measurements. The mrc1Δ or rad9Δ strain was transformed with LEU2 plasmids carrying POL2, pol2-4, or pol2-M644G. Transformants were plated onto FOA media using 10-fold serial dilutions to select for loss of the complementing POL2-URA3 plasmid and photographed after 3 d at 30 °C. (B) Spontaneous mutation rates. Can^r mutants per cell division were determined from multiple independent fluctuation analyses of each strain. The 95% confidence intervals for each mutation rate are shown as error bars. In the grid below the graphs, black boxes designate strain genotypes.