Table 1.
Mutation rates of pol2 shuffling strains
| POL2† | pol2-4† | pol2-M644G† | |||||
| Strain* | Relevant genotype | MR | 95% CI | MR | 95% CI | MR | 95% CI |
| Figs. 2A and 5B‡ | |||||||
| LW14 | WT | 3.0 | (1.8–4.5) | 8.4 | (6.2–11.0) | 30.1 | (25.2–35.3) |
| AH7808 | + sml1Δ | 4.4 | (3.2–5.8) | 13.8 | (11.2–16.8) | 25.9 | (21.4–30.7) |
| AH8009 | + crt1Δ | 2.7 | (1.6–4.3) | 19.4 | (15.1–24.2) | 20.5 | (16.1–25.5) |
| AH8209 | + sml1Δ crt1Δ | 4.8 | (3.7–6.0) | 63.7 | (56.4–71.4) | 42.0 | (36.3–48.2) |
| AH8403 | + dif1Δ | 2.5 | (1.2–4.6) | 18.4 | (13.0–25.0) | 24.9 | (17.0–34.5) |
| AH8604 | + sml1Δ dif1Δ | 7.9 | (5.1–11.5) | 26.0 | (19.5–33.5) | 22.0 | (15.6–29.8) |
| AH9704 | + sml1Δ crt1Δ dif1Δ | 11.6 | (8.2–15.7) | 32.7 | (26.9–39.0) | 54.8 | (42.7–68.4) |
| LW15 | dun1Δ | 1.6 | (0.8–2.9) | 2.3 | (1.4–3.6) | — | |
| AH7905 | + sml1Δ | 3.6 | (2.4–5.2) | 16.6 | (12.4–21.4) | 20.5 | (14.2–28.1) |
| AH8110 | + crt1Δ | 3.1 | (2.0–4.4) | 14.6 | (11.8–17.9) | — | |
| AH8306 | + sml1Δcrt1Δ | 4.3 | (2.8–6.2) | 37.7 | (26.5–51.1) | 32.7 | (22.2–45.9) |
| AH8506 | + dif1Δ | 3.3 | (1.6–5.9) | 4.3 | (2.6–6.8) | — | |
| AH8702 | + sml1Δ dif1Δ | 4.0 | (2.3–6.4) | 17.1 | (11.9–23.6) | 10.7 | (6.9–15.6) |
| AH9806 | + sml1Δ crt1Δ dif1Δ | 6.6 | (4.7–8.9) | 39.7 | (32.6–47.5) | 42.0 | (31.5–54.0) |
| Figs. 2B and 4B‡ | |||||||
| LW14 | WT | 2.9 | (1.0–6.2) | 11.5 | (8.4–15.3) | 33.2 | (25.2–42.4) |
| LW17 | + rev3Δ | 2.2 | (1.1–3.9) | 18.1 | (13.5–23.5) | 53.2 | (42.0–65.5) |
| LW18 | +rad30Δ | 2.8 | (1.7–4.3) | 17.0 | (12.4–22.4) | 52.4 | (41.4–64.6) |
| LW19 | +mrc1Δ | 2.6 | (1.2–4.6) | 12.4 | (8.6–17.2) | 17.1 | (11.9–23.4) |
| LW21 | +rad9Δ | 2.8 | (1.7–4.3) | 18.1 | (13.9–22.8) | 41.2 | (31.6–52.3) |
| Fig. 2C‡ | |||||||
| LW3 | msh6Δ | 38.4 | (30.3–47.4) | 1,370 | (1,200–1,550) | ||
| LW16 | +dun1Δ | 16.1 | (11.6–21.6) | 504 | (445–563) | ||
Mutation rate (MR) and 95% confidence interval (CI) were calculated using Salvador 2.3 with Mathematica 8.0 from the fluctuation of Canr mutants arising in at least three isolates of each strain–plasmid combination (eight replica cultures per experiment), derived from independent plasmid shuffling experiments. These values are multiplied by 1 × 10−7 Canr mutants per cell division. A dash (—) indicates the strain was inviable.
*
Complete genotypes of all strains can be found in Table S1.
†
All plasmids were derived from pRS415 (LEU2).
‡
The figures shown in italics were constructed from the set of mutation rate measurements that immediately follow.