Skip to main content
. 2015 May 5;4:e05505. doi: 10.7554/eLife.05505

Figure 3. Active mechanisms of senescent cell clearance operate in salamander tissues.

(A) Schematic representation of the implantation experiment. 1000 UV-induced senescent or nGFP+ non-senescent cells were implanted within the left or right newt forelimbs, respectively, and analysed at different weeks post-implantation (wpi) by SA-b-gal staining or immunofluorescence (below). Note the complete clearance of senescent cells at 4wpi. Scale bar: 100 µm. (B) Dynamics of senescent cell clearance within adult newt limbs as described in A as shown by a quantification of total cells remaining within entire limbs at different days post implantation (dpi, n = 12). In addition to senescent and nGFP+ control cells, the dynamic of cell clearance was evaluated following implantation of nGFP+ senescent cells, nGFP+ UV-irradiated (1J/m2) cells and a 1:1 mixture of nGFP+ control and senescent cells (nGFP:sen).

DOI: http://dx.doi.org/10.7554/eLife.05505.008

Figure 3.

Figure 3—figure supplement 1. Distribution of senescent cells during limb regeneration.

Figure 3—figure supplement 1.

(A) Quantification of SA-β-gal positive cells after staining of control and UV (10J/m2 or 1J/m2) irradiated nGFP+ cells (12 days post irradiation). (B) Quantification of DHR123 (ROS) positive cells after staining of control and UV (10J/m2 or 1J/m2) irradiated nGFP+ cells (12 days post irradiation).
DOI: http://dx.doi.org/10.7554/eLife.05505.009