Table 1.
Experiment | Mobilising agent | Blood volume (µl)/PBMC # (million–M) | Transfusion time in relation to irradiation (h) | Radiation dose of recipient (Gy) | Recipient % survival at Day 30 | Statistical significance |
---|---|---|---|---|---|---|
1. | TS | Blood 25 µl | +2 | 9.2 | 26 | NS |
Blood 50 µl | +2 | 9.2 | 89 | S | ||
Blood 100 µl | +2 | 9.2 | 84 | S | ||
Blood 150 µl | +2 | 9.2 | 95 | S | ||
Vehicle | Blood 150 µl | +2 | 9.2 | 5 | ||
2. | TS | PBMC 0.5 M | +2 | 9.2 | 84 | S |
Vehicle | PBMC 0.5 M | +2 | 9.2 | 50 | ||
3. | TS | PBMC 2 M | +2 | 9.2 | 100 | S |
Vehicle | PBMC 2 M | +2 | 9.2 | 6 | ||
4. | TS | PBMC 2 M | +24 | 9.2 | 88 | S |
Vehicle | PBMC 2 M | +24 | 9.2 | 50 | ||
5. | TS | Blood 100 µl | +2 | 11 | 100 | S |
Vehicle | Blood 100 µl | +2 | 11 | 31 | ||
TS | Blood 100 µl | +24 | 11 | 88 | S | |
Vehicle | Blood 100 µl | +24 | 11 | 13 | ||
6. | TS | PBMC 5 M | +2 | 11 | 100 | S |
Vehicle | PBMC 5 M | +2 | 11 | 31 | ||
TS | PBMC 5 M | +24 | 11 | 94 | S | |
Vehicle | PBMC 5 M | +24 | 11 | 38 | ||
TS | PBMC 5 M | +48 | 11 | 69 | S | |
Vehicle | PBMC 5 M | +48 | 11 | 0 | ||
7. | TS | PBMC 5 M | +24 | 11.5 | 94 | S |
Vehicle | PBMC 5 M | +24 | 11.5 | 19 | ||
TS | PBMC 5 M | +24 | 12 | 69 | S | |
Vehicle | PBMC 5 M | +24 | 12 | 6 | ||
TS | PBMC 5 M | +24 | 12.5 | 38 | NS | |
Vehicle | PBMC 5 M | +24 | 12.5 | 0 | ||
TS | PBMC 5 M | +24 | 13 | 0 | NS | |
Vehicle | PBMC 5 M | +24 | 13 | 0 | ||
TS | PBMC 5 M | +24 | 14 | 0 | NS | |
Vehicle | PBMC 5 M | +24 | 14 | 0 |
Donor mice were administered TS and AMD3100 (72 and 1 h, respectively) before blood collection. PBMCs were isolated from donor blood samples. Recipient mice (n = 16 per treatment group) were irradiated with varying doses of radiation and then transfused with whole blood or PBMCs at different times after irradiation. S indicates a significant difference between the TS-treated group and the respective vehicle. NS indicates no significant difference.