Fig. 4. pcm¹⁴ larvae are delayed in development.

(A) The growth rate of pcm¹⁴ larvae is not significantly different to wild type (n≥16). (B) Onset of pupariation of pcm¹⁴ larvae is delayed by around 32 hours compared to wild type (n≥42). (C) The mean weight of pcm¹⁴ larvae just prior to pupariation (152 hours) is 120% compared to the mean size of wild-type larvae just prior to pupariation (120 hours). (n≥35, p<0.0001). (D) During the extra 32 hours of development that pcm¹⁴ larvae undergo, the size of the wing imaginal discs increases from 45% to 66% the size of wild type. (n≥30, p<0.001 for all comparisons). Error bars represent 95% confidence limits. (E–G) Wing imaginal disc development in pcm¹⁴ larvae is morphologically delayed by 32 hours as determined by Wingless staining. (E) Wild-type wing imaginal disc at 120 hours displaying the correct pattern of Wingless. (F) pcm¹⁴ wing imaginal disc at 120 hours does not display the correct pattern of expression for this time point. The expression is more diffuse throughout the wing pouch and does not contain the two rings of expression surrounding the wing pouch. (G) pcm¹⁴ wing imaginal disc at 152 hours displaying the pattern of Wingless expression seen in wild type at 120 hours. Scale bars represent 100 µm.