Fig. 8. reaper and hid are post-transcriptionally upregulated in pcm¹⁴ wing imaginal discs as determined by qRT-PCR.

(A) Levels of reaper mRNA increase 7.8-fold in pcm¹⁴ mutant wing imaginal discs compared with wild type whereas levels of pre-reaper RNA are not significantly different. The increase in mature reaper RNA is halved to 3.4-fold in pcm¹⁴;;Df(3L)H99/+ mutant wing imaginal discs, where there is only one copy of the reaper, hid and grim genes. (n = 6 for wild type and pcm¹⁴ and n = 5 for pcm¹⁴;;Df(3L)H99. ***p<0.001. Error bars represent standard error). (B) Levels of hid mRNA increase 2-fold in pcm¹⁴ mutant wing imaginal discs compared with wild type, whereas levels of pre-hid RNA do not differ significantly. The levels of mature hid are reduced in pcm¹⁴;;Df(3L)H99/+ wing imaginal discs, although this difference only showed statistical significance if no correction for multiple comparisons was performed (i.e. if a t-test was used to compare hid levels in pcm¹⁴ and pcm¹⁴;;Df(3L)H99, rather than an ANOVA). (n = 6 for wild type and pcm¹⁴ and n = 5 for pcm¹⁴;;Df(3L)H99. ***p<0.001 and *p<0.05. Error bars represent standard error). (C) Levels of grim mRNA are not significantly different between wild-type and pcm¹⁴ wing imaginal discs. grim pre-mRNA could not be reliably detected. (n = 6 for wild type and pcm¹⁴ and n = 5 for pcm¹⁴;;Df(3L)H99. *p<0.05. Error bars represent standard error). RpL32 (Rp49) was used for normalisation.