Table 6. Cell cytotoxicity of DMs and the templates on NL20 and A549 cell lines.
| Peptide | Percent cytotoxicity ± SD | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NL20 cell line (μg/ml) | A549 cell line (μg/ml) | |||||||||||
| 24 hrs | 48 hrs | 72 hrs | 24 hrs | 48 hrs | 72 hrs | |||||||
| IC50 | Imax | IC50 | Imax | IC50 | Imax | IC50 | Imax | IC50 | Imax | IC50 | Imax | |
| DM1 | 221.8 ± 7.8 | 35.3 ± 6.6 | 235.7 ± 12.9 | 42.2 ± 8.6 | 242.2 ± 7.3 | 45.9 ± 4.3 | 215.3 ± 19.7 | 38.6 ± 6.2 | 195.0 ± 25.2 | 39.8 ± 7.7 | >250 | 66.2 ± 7.2 |
| DM2 | >250 | 64.3 ± 7.0 | >250 | 53.3 ± 9.1 | 241.5 ± 9.0 | 46.8 ± 4.0 | >250 | 50.7 ± 7.7 | 197.7 ± 12.6 | 24.0 ± 6.3 | 165 ± 14.2 | 12.0 ± 4.1 |
| DM3 | 68.5 ± 11.0 | 13.3 ± 2.1 | 91.5 ± 7.6 | 24.0 ± 7.6 | 112.2 ± 8.8 | 12.4 ± 4.5 | 56.0 ± 9.3 | 4.2 ± 3.2 | 80.0 ± 1.7 | 4.4 ± 2.4 | 96.2 ± 4.4 | 3.7 ± 2.1 |
| DM4 | 160.2 ± 11.8 | 6.9 ± 6.8 | 167.3 ± 5.9 | 5.9 ± 2.5 | 205.0 ± 7.0 | 30.2 ± 2.9 | 93.3 ± 5.1 | 8.5 ± 3.8 | 103.0 ± 17.5 | 8.9 ± 1.4 | 107.5 ± 16.3 | 2,9 ± 2.7 |
| DM5 | 179.2 ± 3.8 | 7.3 ± 2.4 | 188.2 ± 4.9 | 8.3 ± 4.2 | 191.7 ± 3.5 | 9.7 ± 5.4 | 97.0 ± 9.8 | 9.0 ± 1.0 | 106.3 ± 15.7 | 7.8 ± 3.8 | 101.7 ± 9.5 | 8.0 ± 3.2 |
| PEN | >4 | 105.2 ± 0.2 | >4 | 106.1 ± 6.2 | >4 | 88.3 ± 0.4 | >4 | 95.0 ± 0.4 | >4 | 97.3 ± 0.4 | >4 | 91.1 ± 6.0 |
IC50 was defined as the concentration of peptide which reduced cell viability to 50%.
Imax was defined as the percentage (%) cell viability tested at the highest concentration of peptides (all peptides - 250 μg/ml; PEN - 4 μg/ml).