FIG. 5.

Effect of in vitro treatment with DBP on cell cycle staging of follicular cells. Antral follicles were mechanically isolated and placed in culture for 24 h and processed for flow cytometry as described in Materials and Methods. Data represent means ± SEM obtained from three separate experiments each with ≥30 follicles per treatment. Representative histograms from vehicle-treated follicles (A) and DBP-treated follicles (B). Summary data from all four experiments (C). Asterisks (*) indicate statistically significant differences (P < 0.05) between DBP-treated follicles and vehicle-treated follicles (DMSO).