FIG. 1.

EGFP transgenic donor cells colonized the seminiferous tubules and established donor-derived spermatogenesis. A) Fluorescent images of whole-mounted seminiferous tubules from a recipient 8 mo posttransplantation. Fluorescent segments are tubules colonized by EGFP-positive donor germ cells. Arrows indicate long stretches of segments with multiple layers of EGFP-positive germ cells, and arrowheads indicate a web of EGFP-positive germ cells. B) EGFP expression was detected in the seminiferous tubules of boar 60 at 3 yr posttransplantation. Boar 60 was a busulfan-treated recipient that received transplantation of germ cells transduced with AAV2-PGK2-EGFP. EGFP driven by the germ cell-specific PGK2 promoter is specifically expressed in spermatocytes and spermatids. C) Control staining without the primary antibody for B. D) Hematoxylin-and-eosin staining of a section from an age-matched, nontreated adult boar testis. Bar = 200 μm (A) and 50 μm (B–D).