FIG. 3.

EGFP transgene is present in IVF embryos produced by sperm from recipient boars. Representative genotyping results from embryos from two recipients (boars 58 and 71) are shown. Two sets of EGFP primers, GFP5′ and GFP3′, were used, resulting in a product of 349 and 230 bp, respectively. Amplification of beta-actin was used as internal control, and the product size was 274 bp. Lanes 1–6: representative embryo samples from recipient boar 58; lanes 7 and 8: representative embryo samples from recipient boar 71; lane 9: embryo from control boar; lane 10: EGFP-positive mouse embryo (positive control); lane 11: no-DNA control; lane 12: 100-bp markers. Boar 58 was a busulfan-treated recipient that received AAV vector-transduced germ cells. Boar 71 was a nontreated recipient that received LV vector-transduced germ cells.