Figure 1.

GluA2-lacking AMPARs are the main contributor to the slow EPSC in hippocampal interneurons. (A) Representative traces of an average EPSC before and after bath application of PhTx. (B1) Isolation of PhTx-resistant (red) and—sensitive (blue) currents overlaid with the current prior to addition of PhTx and normalized to the summed release during the pulse train in baseline conditions. (B2) An expanded view of the tail current is shown. (C) PhTx resistant and sensitive currents were normalized to the total response in the absence of PhTx, and averaged together across multiple cells. Normalization by the total response, defined as defined as the peak response to each stimulus during the train after subtraction of the current immediately preceding that stimulus, summed together over all five stimuli, allows a direct comparison of the slow components in each condition, as this component is the summed response to all five stimuli during the train. (D) PhTx-resistant and—sensitive currents are shown re-normalized to the total response for each respective condition individually, which allows a direct comparison of the kinetics of the slow components in each condition. Inset is an expanded view of tail demonstrating the sensitive current has a slower decay phase.