Figure 3.

Blockade of EAAT1 and EAAT3 recruits a spillover- mediated slow AMPAR tail. (A) Representative average traces are shown for baseline and subsequent application of each drug. (B) The change in average ratio of the late EPSC relative to the charge transfer for the early EPSC is shown normalized to baseline. (C) Average group EPSCs normalized to baseline total response are shown after addition of each drug. 0.5 μM WAY213613 had a minimal effect while 10 mM TBOA had an appreciable observed enhancement of the tail, suggesting EAAT1 is the major glutamate transporter at this synapse. (D) Exponential fitting found that both 10 and 100 μM TBOA increased the relative proportion of the slow component. However, only the highest dose reliably recruited a third, very slow current.