FIG. 3.

Obesity impacts protein abundance of ovarian steroidogenic enzymes. At the end of each experimental time point (6, 12, 18, and 24 wk of age), ovaries were removed from lean (L) or obese (O) female mice, and total ovarian protein was isolated (n = 3–4 per treatment group per time point). Western blotting was performed to measure STAR (A), CYP11A1 (B), 3β-HSD (C), and CYP19A1 (D) protein levels. Densitometry of the appropriate bands was performed using Carestream molecular imaging software and normalized to Ponceau S staining prior to statistical analysis. Bars represent means ± SEM; *significant differences from respective lean group at P < 0.05; † = P < 0.1. Ponceau S loading control is indicated by LC.