FIG. 4.

Ovarian NF-κB pathway member protein levels are affected by obesity. At each time point (6, 12, 18, and 24 wk of age), total ovarian protein was isolated from both lean (L) and obese (O) female mice. Western blotting was performed to quantify pIKKα/β^Ser176/180 (A), pIκB^Ser32/36 (B), and pNF-κB p65^Ser536 (C) protein levels. Densitometry of the appropriate bands was performed using Carestream molecular imaging software and normalized to Ponceau S staining prior to statistical analysis. Bars represent means ± SEM; *significant differences from respective lean group at P < 0.05. Ponceau S loading control is indicated by LC.