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. 2014 Sep 10;91(4):96. doi: 10.1095/biolreprod.114.121434

FIG. 1.

FIG. 1

Proteins identified to be present in the mitochondria ETC BN-PAGE protein complexes. Isolated mitochondria from control and hCG-treated MA-10 cells were solubilized and run on a BN-PAGE gel. The gel was divided into the indicated molecular weights (MWs) and analyzed via mass spectrometry. Identified proteins were grouped via MitoMiner to be present in the ETC complexes in both control (A) and hCG-treated (B) isolated mitochondria. The differences in the proteins identified from hCG-treated minus the control (C) samples demonstrate a larger-molecular-weight molecular complex containing complexes I, III, and V at MW 1048. Proteins identified to be present in complex I: Ndufa7, Ndufa8, Ndufs1, Ndufb5, Ndufa5, Ndufaf1, Ndufa2, Ndufb1, Ndufa9, Ndufa6, Ndufa13, Ndufs4, Ndufs3, Ndufs7, Ndufv1, Ndufb4, Ndufa10, Ndufa12, Ndufs2, Ndufc2, and Ndufv3. Complex III: Uqcr10, Uqcrc2, Uqcc, Uqcrc1, Uqcrfs1, Uqcrq, and Uqcrb. Complex IV: Cox16, Cox5b, Cox4i1, Cox5a, Cox15, Cox7c, Cox7a2, Cox6b1, and Cox7a1. Complex V: Atp5b, Atp5c1, Atp5o, Atp5h, Atp1a2, Atp5a1, Atp5f1, Atp5i, Atp5l-ps1, Atp5g1, Atp5j2, Atp5l, Atp2a2, Atp2a1, Atp1a1, Atp1b3, Atp13a1, Atp5d, Atp6v0d1, Atp5j, Atp5k, Atp6v1e1, Atp6v1a, Atp6v1b1, Atp6v1b2, and Atp6v1h.