FIG. 10.

Differential expression of FSHR in human myometrial stroma of nonpregnant versus pregnant (term) uterus. Tissues in A (nonpregnant myometrium) and B (pregnant myometrium at 38–40 wk gestation) were stained with antibody FSHR-323 IgG2a (brown) and counterstained with hematoxylin (blue) and are shown at magnification ×200. Labeled are myometrial muscle fibers (M with bracket), myometrial blood vessels (V), and arterial smooth muscle (AM). Images in A and B are representative of samples from three nonpregnant women and three pregnant (term) women, respectively. Corresponding negative controls stained with nonimmune IgG2a at the same concentration are shown in Supplemental Figure S10. C) Quantitation of FSHR expression in muscle (excluding that in blood vessels) from all of the nonpregnant and pregnant (term) samples, based on immunohistochemical staining of all slides in the same experiment and quantification as described in Materials and Methods. Data shown are the mean ± SEM. Asterisk denotes a difference of P < 0.05.