TABLE 1.

Efficiency of four different CRISPRs plasmids (guides 10, 131, 256, and 282) targeting CD163. Four different CRISPRS were tested at an amount of 2 μg to 1 μg donor DNA (shown in Supplemental Fig. S1).

^*Mix of 4 + donor DNA represents a mix of 0.5 μg of each CRISPR guide with 1 μg of donor DNA. The donor DNA treatment served as the no CRISPR control, and the 10 + Neo treatment illustrates that the large deletions observed in the CRISPR treatments were present only when the CD163 donor DNA was also present.

^†ANOVA was performed comparing the average number of colonies/plate to estimate CRISPR toxicity and on the percent colonies with a modified genome. P values were 0.025 and 0.0002, respectively. n/a = There were no replicates for this treatment so no statistical analysis was performed.

^‡The one colony with HR represents a partial HR event.

^a–cSuperscript letters indicate a significant difference between treatments for both average number of colonies/plate and percent colonies with a modified genome (P < 0.05).