TABLE 1.
Mix of 4 + donor DNA represents a mix of 0.5 μg of each CRISPR guide with 1 μg of donor DNA. The donor DNA treatment served as the no CRISPR control, and the 10 + Neo treatment illustrates that the large deletions observed in the CRISPR treatments were present only when the CD163 donor DNA was also present.
ANOVA was performed comparing the average number of colonies/plate to estimate CRISPR toxicity and on the percent colonies with a modified genome. P values were 0.025 and 0.0002, respectively. n/a = There were no replicates for this treatment so no statistical analysis was performed.
The one colony with HR represents a partial HR event.
Superscript letters indicate a significant difference between treatments for both average number of colonies/plate and percent colonies with a modified genome (P < 0.05).