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Efficiency of CRISPR/Cas9-induced mutations without drug selection. Four different ratios of donor DNA to CRISPR 134 DNA were compared in a previously modified cell line without the use of G418 selection.
Significant difference between treatments for percent colonies with NHEJ repair (P > 0.05).
There was not a significant difference in the number of genome-modified colonies with increasing concentration of CRISPR (P > 0.33).
