Table 1.

Expression of differentiation and activation markers on re-stimulated CD4+ cells

	IFN-g, % pos	IL4, % pos	CD25, % pos
CD19-Fc, 1:100	3.0	2.3	10.3
ephrin-A4-Fc, 1:100	1.9	1.4	6.1
CD19-Fc, 1:10	10.2	2.6	19.3
ephrin-A4-Fc, 1:10	7.4	2.1	17.3

The reduction in T cell proliferation is not a result of a change in the Th1/Th2 ratio, but rather a general reduction in the activation status of the T cells. Resting CD4+ cells were stimulated one week with LLDC, in the presence of immobilized control protein or ligand, and re-stimulated 5 hours with TPA and ionomycin. One of three representative experiments is shown, and data represent percent of living cells staining the indicated antibody.