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Primers and probes used in this study.
| Gene | Primer | Primer sequence (5′ → 3′) | Probe sequence | Reference |
|---|---|---|---|---|
| ssl8 | Forward | CCAACAAAATCAAAAGTTCCA | FAM-CACATGGTTTAGATGT CTTTGCGGTACC-NFQ | This study |
| Reverse | CACCACTAACACTAAATATTCTTCCA | This study | ||
| Gmk | Forward | ACTAGGGATGCGTTTGAAGCTTTAA | FAM-AAAGATGACCAATTT ATAG AATATG-NFQ | This study |
| Reverse | ACCATAATAGTTGCCTACAT | This study | ||
| RNAIII | Forward | TCCATTTTACTAAGTCACCGATTGT | FAM-ATCTTGTGCCATTGAAATCACTCCTTCCTT-NFQ | 33 |
| Reverse | TGTGATGGAAAATAGTTGATGAGTTGT | 33 |
