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PCR-RFLP analysis of the CYP2D6*10 allele using the HphI enzyme.
Notes: Lane 1 indicates the PCR product; This enzyme generated four fragments of 362 bp, 262 bp, 100 bp, and 71 bp (lane 2) in the heterozygous C/T genotype; three fragments of 262 bp, 100 bp, and 71 bp (lane 3) in the homozygous T/T genotype; and two fragments of 362 bp and 71 bp (lane 4) in length in the wild-type C/C genotype.
Abbreviation: PCR-RFLP, polymerase chain reaction-restriction fragment length polymorphism.
