FIG. 1.

Dex and E₂ regulate global gene expression in human uterine epithelial cells. A) mRNA isolated from Dex-, E₂-, and Dex + E₂-treated ECC1 cells was analyzed using Whole Human Genome 4x44 multiplex format oligo array (Agilent) for gene expression. A heat map was generated with Heatplus software (BioConductor), using the averaged, normalized sample replicates of all significant probes. The number of probes that were statistically different (P < 0.01) between treatment groups were sorted by Venn diagram. B) The number of probes that were regulated are organized as either induced or repressed according to treatment group in Dex, E₂, and Dex + E₂ samples. C) The top five induced and repressed genes for each treatment group are listed. D) IPA of the top five regulated networks using the gene lists generated from Dex-, E_2-, and Dex + E₂-treated ECC1 cells. E) Embryonic development and organ development network is presented, with expression values of the Dex, E₂, and Dex + E₂ treatment groups overlaid, as a colorimetric indicator of up- or down-regulation. F) Independent validation of Left-Right Determination Factor 1 (LEFTY1), one of the antagonized genes identified in the embryonic development and organ development network. Values were normalized to the housekeeping gene Cyclophilin B. Bar graphs show means ± SEM. **Groups with statistically different means at P < 0.01, as determined by ANOVA.