FIG. 4.

Comparison of coregulated genes reveals several targets of Dex and E₂ antagonism. A) Dex-, E₂-, and Dex + E₂-coregulated genes were separated by direction of regulation. Anticorrelated genes were classified as opposing directions of regulation (positive vs negative). Antagonized genes were classified as genes with greater than two-fold difference between treatments. B) Genes classified as regulated by Dex and Dex + E₂ were separated by direction of regulation. Anticorrelated genes were classified as opposing directions of regulation (positive vs. negative). Antagonized genes were classified as genes with greater than 2-fold difference between treatment type compared to Dex + E₂. C) Genes classified as regulated by E₂ and Dex + E₂ were separated by direction of regulation. Anticorrelated genes were classified as opposing directions of regulation (positive vs. negative). Antagonized genes were classified as genes with greater than two-fold difference between treatment type compared to Dex + E₂. D) Guanosine monophosphate reductase (GMPR) represents the pattern of expression found for Dex and Dex + E₂ antagonistically regulated genes. RNA Binding Motif Protein 24 (RBM24) represents one gene identified as antagonistically regulated by E₂ and Dex + E₂. G Protein-Coupled Estrogen Receptor 1 (GPER) and Chromosome 10 Open Reading Frame 10 (C10orf10) illustrate differentially regulated genes with additive expression values.