Table 1.

Gene specific amplification primers

Target Gene	Primer pair sequences (5' – 3')	Accession number	Position in sequence	Fragment Size (bp)	Annealing Temp (\r C)
β actin	GACAGCAGTCGGTTGGACC	M10277	3163–3179	387	62
	CAGGTAAGCCCTGGCTGC		3549–3532
Cx26 (a)	GTTTAACGCATTGCCCAGTT	M86849	874–893	174	62
	TGTGGCATCTGGAGTTTCAC		1047–1028
Cx26 (b)	AGGCCTGTCCAACACATCTC		1760–1779	244	64
	AGGGGGTAAGCCAAACAAAC		2003–1984
Cx31	TGCAGTGGAGAGGAGGTCTT	BC012918	1454–1473	184	66
	CAGTTGTGGGGAGGAAGATG		1637–1618
Cx32 (a)	GGGTACAAGAGATGGGATGC	BC039198	1200–1219	202	64
	GCCAGCAAGCACTATTCCTC		1401–1382
Cx32 (b)	CCCTGGTTTTCTGGAGTCAC	NM_000166	1273–1292	206	62
	CCCTGCTCCAACTTATCTGC		1478–1459
Cx40 (a)	TTGCAACCTTTCCTTCTGCT	BC013313	1878–1897	180	64
	GTGAACAGCCAAGGGAGAAA		2057–2038
Cx40 (b)	CCCTGCTAGGGAGTCACTGT		1929–1948	201	62
	CTGGTCAGGGTTCGAGAGAG		2129–2110
Cx43	CTGACATGCATGCAAGAAGAA	BC026329	2802–2782	217	64
	TCTTTTGGAGTGACCAGCAA		2586–2605
Cx45	AGATCAGGATGGCTCAGGAA	U03493	1022–1041	155	64
	GTTCTTCCCATCCCCTGATT		1176–1157