Table 4.
Embryo | Cx43 | Cx26 | Cx31 | Cx45 | Cx40 |
1 | + | + | |||
2 | + | + | |||
3 | + | + | |||
4 | + | + | |||
5 | - | - | |||
6 | + | + | |||
7 | + | - | |||
8 | + | + | |||
9 | - | - | |||
10 | - | - | |||
11 | + | - | |||
12 | + | + | |||
13 | + | + | |||
14 | + | + | |||
15 | + | + | |||
16 | - | - | |||
17 | + | - | |||
18 | + | - | |||
19 | + | - | |||
20 | + | - | |||
21 | - | - | |||
22 | - | - | |||
23 | + | - |
Individual human blastocysts were stained with antibodies to Cx 43,26, 31, 45 and 40 as indicated in materials and methods. Double staining with Cx43 and one other connexin was carried out in each case. +, specific staining detected; -, no staining detected.